Fig. 4

WSB-1 regulates angiogenic potential in vitro. a MDA-MB-231, MDA-MB-468, MCF7, and T47D cells were transfected with WSB-1 (siWSB-1) or non-targeting siRNA (siNT), and exposed 24 h to 20% or 2% O₂. VEGF levels in conditioned media were quantified by ELISA. Histogram represents average of n = 3 experiments. b Angiogenic potential was assessed using in vitro branch forming assays using conditioned media from MDA-MB-231 cells transfected with siWSB-1 or siNT. Histogram represents average of n = 3 experiments. c Inset microscopy images are representative of each condition. Scale bar represents 200 μm. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001