Fig. 5

WSB-1 regulates invasiveness of HR-negative cells in vitro MCF7 and MDA-MB-231 cells were transfected with siWSB-1 or siNT. a Cells were seeded in control (uncoated) or matrigel-coated transwell inserts and allowed to invade for 18 h at 20% or 2% O₂. Invasion index = % invasion siWSB-1/% invasion siNT. Histograms represent n = 4 experiments. b Transcript levels of MMP1 and MMP14 were assessed after 24 h exposure to 20% or 2% O₂. Histograms represent average of n = 3 experiments. c MDA-MB-231 cells were transfected with either siWSB-1or siNT, and exposed to 20% or 2% O₂ for 24 h. Whole cell lysates (WCL) or conditioned media samples were analysed by Western blotting for the expression of HIF1α, MMP14, MMP2, and MMP1. Gelatine zymography was used to determine the activity of MMP2 in conditioned media samples. Representative blots and zymograms are shown (n = 5). *p < 0.05; **p < 0.01; ***p  <  0.001;****p < 0.0001