Table 4 Summary of studies that reported on heterogeneous MYCN amplification in NB
From: Heterogeneous MYCN amplification in neuroblastoma: a SIOP Europe Neuroblastoma Study
Study | n cases (%) | Technique | Sample (n cases) | HetMNA | Age >/<18 months (n) | Stage (n) | Outcome (n) | |
|---|---|---|---|---|---|---|---|---|
Patterns | Features (MYCN copies) | |||||||
Squire et al., 1996 | 5/29 (17.2) | FISH, SB, PCR | PT | Intratissue | 2/5 with small number of MNA cells (3–10) | NR | NR | NR |
Lorenzana et al., 1997 | 1 | FISH, SB | PT & CTX | Intratissue | GNB: MNA u-nbs (15–20), nonMNA d-nbs | > | 3 | DOD |
Ambros et al., 2001 | 3/300 (1) | FISH | PT | Intratissue | Focal MNA (10–50) | NR | 1 (2), 4s (1) | NR |
Kerbl et al, 2002 | 1 | FISH | PT | Intratissue | Focal MNA (>15) | < | NR | ADF |
Noguera et al., 2003 | 1 | FISH | PT & R | Temporal | PT: MNG, R: homMNA | < | 4s | DOD |
Valent et al., 2004 | 4/200 (2) | FISH, Q-PCR | PT (4), BM (1) | Intratissue & spatial | MNA and MNG cells; PT: homMNA, BM: MNG | NR | 4s (2), 3 (1), 4 (1) | ADF (1) AWT (2) DOT (1) |
Spitz et al., 2004 | 4/659 (<1) | FISH | PT (2), BM (1), CTX (1) | Intratissue, spatial & temporal | Focal and scattered MNA; PT/BM: hetMNA, R: homMNA; PT: nonMNA, BM: MNA | NR | NR | NR |
Thorner et al., 2006 | 4/41 (9.7) | CISH, FISH, SB, PCR | PT | Intratissue | ≥50% MYCN copy number difference from cell to cell | NR | NR | NR |
Sano et al., 2007 | 1 | FISH | PT | Intratissue & spatial | Composite NB: noMNA, MNA; Lymph nodes: homMNA | < | 3 | AWT |
Cañete et al, 2009 | 5/46 (10.9) | FISH | PT | Intratissue | SIOPEN cohort | < | NRa | NR |
Theissen et al., 2009 | 20/1341 (1.5) | FISH | PT (15), PT & BM (3), PT & R (2) | Intratissue, spatial & temporal | Focal & scattered MNA; PT: hetMNA, R: nonMNA/hetMNA/homMNA; PT: nonMNA/homMNA, R:hetMNA/homMNA; PT: nonMNA, BM: nonMNA/hetMNA, R: hetMNA/homMNA | < (9) & > (11) | (2), 4s (3), 3 (6), 4 (9) | ADF (7) Prog. (4) DOD (9) |
Bishop et al., 2014 | 1 | FISH | PT | Intratissue, spatial | Focal MNA; adrenal mass: hetMNA, Liver: homMNA | < | 4s | ADF |
Berbegall et al., 2016 | 3 | FISH, aSNP | PT | Intratissue, spatial & temporal | Iliac crests: nonMNA and MNA; BM: nonMNA, metastases: hetMNA | > | 4 | Prog. |
Marrano et al., 2017 | 8/30 (26.7) | qPCR, FISH, CISH | PT/CTX (4), PT & R (1), M (1), CTX & R/M (2) | Intratissue, spatial & temporal | Changes in the MYCN copy number (reduction or increase) | < (2) & > (5) | (6) | DOD (6), A (2) |
