Fig. 1

Increased expression of miR-1246 in exosomes secreted by irradiated BEP2D cells and inhibition of cell proliferation. a Representative image of exosomes using transmission electron microscopy. The exosomes were isolated from the culture medium of 2 Gy-irradiated BEP2D cells collected at 4 h postirradiation. b Western blot analysis of exosomal marker proteins. c Changes in expression of miR-1246 in exosomes of γ-ray-irradiated BEP2D cells. Exosomes were isolated from the culture medium of 2 Gy-irradiated BEP2D cells or control nonirradiated cells at 1−24 h postirradiation. miRNA expression levels in the exosomes were detected by qPCR. miR-1246 expression data were normalised using miR-16 as an internal control as described previously.⁵⁴ #p < 0.01 as compared with the expression of miR-1246 in exosomes from nonirradiated control cells at the same timepoint. d Expression of intracellular miR-1246 was detected in BEP2D cells at 1 and 24 h post 2 Gy irradiation by qPCR. The RNA U6 was used as an internal control for intracellular miR-1246. #p < 0.01 as compared with the expression of miR-1246 in nonirradiated control cells. e Inhibition of miRNA-1246 mimic on BEP2D cell proliferation. BEP2D cells were transfected with 50 nM miRNA-1246 mimic or miR-NC and cell proliferation was evaluated using the CCK-8 assay at 48 h after transfection. *p < 0.05 as compared with control miR-NC-treated cells. f Enhanced proliferation of BEP2D cells by miRNA-1246 inhibitor. BEP2D cells were transfected with 50 nM miRNA-1246 inhibitor or inhibitor-NC and cell proliferation was evaluated using the CCK-8 assay at 48 h after transfection. *p < 0.05 as compared with control inhibitor-NC treated cells. g Decreased colony-forming ability of BEP2D cells by miRNA-1246 mimic. BEP2D cells were transfected with 50 nM miRNA-1246 mimic or miR-NC and colony-forming ability was assayed following miRNA transfection. *p < 0.05 as compared with control miR-NC-treated cells