Fig. 2

Increased yield of micronuclei in BEP2D cells transfected by miR-1246 mimics and radiation conditioned cell culture medium (RCCM). a Representative image of a micronucleus detected by DAPI staining. b Increased micronuclei in BEP2D cells following transfection with miR-1246 mimic. Micronuclei were scored in BEP2D cells following treatment with miR-1246 mimic or control miR-NC for 48 h. In total, 500 randomly selected cells were scored in each group (total number of micronuclei). *p < 0.01 as compared with control miR-NC cells group. c Induction of micronuclei in BEP2D cells by RCCM. RCCM was collected from 2 Gy γ-ray-irradiated BEP2D cell cultures at 4 h postirradiation. The nonirradiated control cell culture medium (CCCM) was used as a control. BEP2D cells were cultured in CCCM or RCCM with or without miR-1246 inhibitor or NC-inhibitor control. The micronuclei were scored 48 h later using DAPI staining. In total, 500 randomly selected cells were scored in each group (total number of micronuclei). *p < 0.01 as compared with miR-NC or NC-inhibitor control group. d, e Effects of miR-1246 mimic (d) or inhibitor (e) on the radiation sensitivity of BEP2D cells. In total, 5000 BEP2D cells were seeded in each well of 96-well plates. Following transfection of miR-1246 mimic, inhibitor, or NC controls for 24 h, cells were irradiated with 2 Gy of γ-rays. Proliferation was measured with the CCK-8 assay at the indicated timepoints after 2 Gy irradiation. *p < 0.01 as compared with the miR-NC group