Fig. 3
From: Exosome-packaged miR-1246 contributes to bystander DNA damage by targeting LIG4
Increased induction of 53BP1 foci of DNA double-strand breaks (DSBs) in BEP2D cells by exosomes, RCCM, and miR-1246. a 53BP1 foci of DSBs in BEP2D cells induced by IR-exosomes from 2 Gy-irradiated BEP2D cells isolated at 4 h postirradiation. Exosomes were isolated from culture medium (3.8×109 particles per 10 mL) (Supplementary 1B and C). b Quantitative determination of 53BP1 foci in BEP2D cells following treatment of IR-exosomes for 24 h with or without miR-1246 inhibitor. In total, 50 randomly selected cells were scored in each group (mean ± standard deviation (SD)). *p < 0.01 as compared with con-exosomes isolated from nonirradiated BEP2D cells. #p < 0.01 as compared with IR-exosomes. c 53BP1 foci of DSBs in BEP2D cells induced by RCCM of Gy-irradiated BEP2D cells collected at 4 h postirradiation. d Quantitative determination of 53BP1 foci in BEP2D cells cultured in RCCM or CCCM for 24 h. In total, 50 randomly selected cells were scored in each group (mean ± SD). The exosome-free RCCM was the RCCM supernatant following the removal of exosomes by ultracentrifugation. *p < 0.01 as compared with the CCCM of nonirradiated control cell culture medium. #p < 0.01 as compared with the RCCM group. e 53BP1 foci of DSBs in BEP2D cells induced by miR-1246 mimic. f Quantitative determination of 53BP1 foci in BEP2D cells following treatment with miR-1246 mimic for 24 h. In total, 50 randomly selected cells were scored in each group (mean ± SD). *p < 0.01 as compared with the control miR-NC. g 53BP1 foci of DSBs in HEK-293T cells induced by miR-1246 mimic. f Quantitative determination of 53BP1 foci in HEK-293T cells following treatment with miR-1246 mimic for 24 h. In total, 50 randomly selected cells were scored in each group (mean ± SD). *p < 0.01 as compared with the control miR-NC
