Fig. 3
From: Tumour-infiltrating neutrophils counteract anti-VEGF therapy in metastatic colorectal cancer
Impact on hypoxia, cell death and neutrophil invasion in colorectal cancer xenografts. a Images (40×) of hypoxic tumour area (pimonidazole adducts, green), tumour cell death (cl. caspase-3, red) and adjacent blood vessels (CD31, purple). b Quantification of overall tumour hypoxia c haematoxylin and eosin (H&E) staining of tumour sections depicting necrotic tumour area and d quantification of tumour cell death and e cell death/hypoxia ratio. f Images of xenograft-infiltrating neutrophils (Ly6G/C red, 100×) and g quantification thereof; h quantification of tumour-infiltrating neutrophils in the transition zone from hypoxia to necrotic tissue. *p < 0.05, **p < 0.01, n.s. not significant
