Table 1 Selected factors (mRNA/protein) differentially regulated in OV-KLK4–7 versus control OV-VC cells as assayed via PCR arrays, microarray and secretome analysis

From: Kallikrein-related peptidases 4, 5, 6 and 7 regulate tumour-associated factors in serous ovarian cancer

Gene

Protein

PCR array

Microarray

Secretome

MSN

Moesin

396.2

53.6

7.1

KRT19

Keratin, type-I cytoskeletal 19

347.3

186.6

8.8

COL5A2

Collagen alpha-2(V) chain

71.0

6.4

30.5

COL1A2

Collagen alpha-2(I) chain

69.6 a

9.2

7.9 b

BMP5

Bone morphogenetic protein 5

20.3

4.6

20.8 b

F10

Coagulation factor X

10.6

6.4

17.9 b

KRT7

Keratin, type II cytoskeletal 7

−1038.3

−16.5

−2.1 b

JUNB

Transcription factor jun-B

−12.1 a

−1.0

n.d.r.

BMP4

Bone morphogenetic protein 4

−12.2

−3.2

n.d.r.

MMP1

Interstitial collagenase

−12.1

1.9

−1.7b

  1. PCR array and microarray analyses were performed using mRNA extracted from cell lysates, whereas cell-conditioned media were used for secretome analysis. The secretome profiling data were added from Shahinian et al.14. Bold numbers indicate upregulation and italic numbers downregulation (fold change; n.d.r. not differentially regulated).
  2. aCOL1A2 and JUNB were present on two different PCR arrays and found to be differentially regulated in both cases; highest/lowest fold change listed
  3. bMSN, KRT19 and COL5A2 were differentially regulated in all three replicate secretome samples; the other proteins mentioned were differentially regulated in one or two replicates. Except for JUNB and BMP4 (n.d.r.)
  4. MSN, KRT19, KRT7 and JUNB, underlined, were chosen for further analyses via qPCR, western blotting, immunofluorescence and immunohistochemistry (see Figs. 2–4)
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