Fig. 6

Double immunofluorescence showing Wt1 expression in the breast tissue of C3(1)/Tag mammary tumour mice versus Friend Virus B-type (FVB) controls. a Some epithelial cells of the terminal duct lobular unit (TDLU) were Wt1-positive (green) in the healthy FVB mouse. b A blood vessel within the mammary tissue of the control FVB mouse-positive for the endothelial cell marker, CD31 (red) and co-localising with Wt1 (green). c The tumour stroma of the C3(1)/Tag mammary carcinoma model exhibits global Wt1 (green) expression, whilst d depicts Wt1-positive (green) endothelial cells (red) at higher magnification in the same animals (note DAPI = Blue in all images). Compared with controls, tumours from the C3(1)/Tag mice exhibited higher numbers of: e Wt1-positive cells, f CD31-positive cells, g Wt1/CD31 double-positive cells, h total blood vessels, and i Wt1-positive blood vessels. j The percentage of Wt1-positive blood vessels was also increased in the C3(1)/Tag tumour model when compared to controls. Data are expressed as median ± interquartile range, where *p < 0.05 and **p < 0.01 by Mann–Whitney U test (n = 6)