Fig. 3 | British Journal of Cancer

Fig. 3

From: (Pro)renin receptor promotes colorectal cancer through the Wnt/beta-catenin signalling pathway despite constitutive pathway component mutations

Fig. 3

(P)RR silencing attenuates the activation of Wnt/β-catenin signalling in CRC cells. a, b Representative western blotting images showing protein levels of components of the Wnt/β-catenin pathway in DLD-1 cells with mutated APC (a) and HCT116 cells with mutated β-catenin (b) after siRNA transfection. c, d Relative mRNA levels of components of the Wnt/β-catenin pathway in DLD-1 (c) and HCT116 (d) cells after siRNA transfection. Values were calculated from signal intensities obtained by real-time PCR (n = 3~6). Data are expressed as relative ratios to the scrambled siRNA group. e, f Wnt/β-catenin signalling activity of DLD-1 (e) and HCT116 (f) cells transfected with β-catenin-responsive TOP flash reporter and siRNA (n = 4). Data are expressed as relative ratios to vehicle (only transfected with the TOP flash reporter without siRNA) group. Cells transfected with the FOP flash reporter, of which the TCF/LEF binding sites were mutated, served as negative control. Numerical data are presented as mean ± SEM. N.S.: not significant, *P < 0.05, **P < 0.01, ***P < 0.001

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