Fig. 1

Mass spectrometry analysis of the PRICKLE1 protein complex from a TNBC cell line. a Schematic representation of the proteins associated to PRICKLE1 identified by mass spectrometry analysis from MDA-MB-231 cell extracts. Proteins have been classified following their function in several groups: Small G-proteins regulators, cytoskeleton-associated, kinases, membrane proteins, proteins involved in ubiquitination, scaffold proteins, and others. b Volcano plot showing the significance two-sample t-test (−Log p value) vs. fold-change (Log2 (GFP-PRICKLE1 vs. GFP as control)) on the y and x axes, respectively. The full line is indicative of protein hits obtained at a permutation false discovery rate of 1% (pFDR). Data results from two different experiments processed three times. PRICKLE1 (the bait) is represented in red and ECT2, one of the most abundant PRICKLE1-associated partners, is represented in green