Fig. 4

Prickle1 and Ect2 functionally interact in Xenopus during embryonic development. In situ hybridisation against ect2 transcripts at stage 8, 9, and 10. ect2 RNA is detectable in the animal pole (animal view and lateral view) but not in the vegetal pole (vegetal view) at stages 8 and 9, but no longer at stage 10. Schematic representations of embryos at the stages analysed are shown on the right. b Embryos at two-cell stage were injected into two blastomeres with Prickle1 and Ect2 MOs as indicated. In all cases 0.5 ng of mRFP mRNA was injected as control and tracer. Suboptimal doses (10 ng) of either MO did not cause CE problems. However, when both Prickle1 and Ect2 MOs were co-injected at suboptimal doses (5 ng each), embryos displayed CE problems at a rate comparable to high doses of each MO injected separately (40 ng Prickle1-MO or 20 ng Ect2-MO). A total of 60 embryos per condition was analysed in two independent experiments. Pictures illustrate representative phenotypes. SR survival rate, ND percentage of surviving embryos developing normally, CED, percentage of surviving embryos showing convergent-extension defects. Scale bars: a = 0.25 mm; b = 0.5 mm