Fig. 4

Knockdown of PPM1H in CRC cells increased VIM expression in fibroblasts and its proliferation, and activated fibroblasts facilitated CRC cell proliferation and invasion. (a) VIM mRNA or protein expression of CCD-18Co with respect to the different CMs from the indicated CRC cells after siPPM1H or siControl interference; (b) proliferation of CCD-18Co with respect to the different CMs from the indicated CRC cells after siPPM1H or siControl interference; (c) proliferation of SW480 and CaCO2 cultured with the different CMs from activated CCD-18Co and CCD-18Co-control; (d) cell invasion of SW480 and CaCO2 cultured with different CMs from activated CCD-18Co and CCD-18Co-control. CMs, conditional media. Each assay was repeated thrice