Fig. 4

Crenolanib inhibits A2780 tumour growth and PDT-induced sprouting in the CAM model. a Tumour growth curves of A2780 spheroids xenografted on the CAM. Tumour volume is represented as the percentage of control tumour volume in respect to treatment days. n = 41 in the control group and n = 12–27 in the treatment groups. Eggs were treated with vehicle (0.1% DMSO) or crenolanib (52 µg/kg/day and 260 µg/kg/day) 2x daily i.v. injections. b Final tumour volume at the day of tumour resection. c Representative images of tumour sections stained for CD31. d Representative images of tumour sections stained for Ki67. e Microvessel density analysis of tumour sections stained for the endothelial cell marker CD31. f Analysis of the fraction of Ki67 positive cell nuclei presented as the percentage of stained cells per image field. g Effect of crenolanib at a dose of 63.5 µg/kg on PDT-induced vascular sprouting in the CAM. Quantification of the number of sprouts and the average sprout length are shown as percentage of control. Significance (*P < 0.05, **P < 0.01) is indicated as compared to control (0.1% DMSO treated cells) and error bars indicate SEM. Representative images 24 h after PDT are shown. The bottom panel indicates zoomed areas corresponding to yellow boxes in the upper panel