Fig. 4 | British Journal of Cancer

Fig. 4

From: Acidity promotes tumour progression by altering macrophage phenotype in prostate cancer

Fig. 4

Buffering tumour-secreted acids alters tumour associated macrophage (TAM) phenotype in vivo and reduces tumour progression. a Inflammation score and b Volcano plot generated by the nSolver software 3.0 using gene expression data from nCounter PanCancer Immune profiling of CD11b+F4/80+ TAMs sorted from control or sodium bicarbonate-treated (buffered) TRAMP-C2-bearing mice (n = 4–5). c Fold expression of Cd206 and Arg1 in CD11b+F4/80+ TAMs sorted from independent cohort of control or sodium bicarbonate-treated (buffered) TRAMP-C2-bearing mice (n = 5–10). d Histopathological analysis of haematoxylin & eosin (H&E) samples from 6-, 15-, 22-, 23- and 25-week-old TRAMP mice. e, f Quantification of F4/80 (macrophage) and α-smooth muscle actin (fibroblast) staining in serial sections of paraffin-embedded prostates, isolated from 6-, 15-, 22-, 23- and 25-week-old TRAMP mice. g TRAMP mice were treated with sodium bicarbonate buffer starting from 4 weeks of age (buffered) or kept on tap water as control. F4/80-stained sections were digitally quantified and the percentage of F4/80 staining intensity in stroma of prostate tissue were plotted (n > 4). h Mean area of segmented stromal compartment (n = 4). i Histopathological analysis of H&E slides of buffered and control TRAMP mice. Data are presented as mean ± SEM. Student’s t test was utilised for statistical analysis; *p < 0.05, **p < 0.01

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