Fig. 2

TRIP13 is required for the proliferation of GBM cells. a Western blot and quantitative PCR assays were used to characterise the expression of TRIP13 in TRIP13-knockdown U87MG and LN229 cells. b MTT assay was performed to examine the proliferative ability of TRIP13-knockdown U87MG and LN229 cells. c BrdU incorporation assays were performed to detect the amount of DNA synthesis in TRIP13-knockdown U87MG and LN229 cells. d The cell cycle was analysed in U87MG and LN229 cells by flow cytometry. e, f Western blot and quantitative real PCR assays were used to detect the expression of cell cycle-related proteins and mRNA levels in TRIP13-knockdown U87MG and LN229 cells