Fig. 5
TRIP13 regulates the expression of c-MYC by reducing c-MYC ubiquitination. a Cell lysates were prepared from TRIP13-knockdown cells that had been treated with or without MG132 for 7 h. Equal amounts of cell lysates were immunoblotted with the indicated antibodies. b The c-MYC turnover rate of TRIP13-overexpressing cells is shown. U87MG and LN229 cells were transfected with TRIP13 plasmid and then treated with CHX (100 μg/ml) for the indicated times. Cell lysates were immunoblotted with the indicated antibodies. c Transfected 293FT cells were treated with MG132 for 7 h before proteins were harvested. The ubiquitinated c-MYC proteins were pulled down with an anti-c-MYC antibody and immunoblotted with an anti-HA antibody
