Fig. 6
TRIP13 regulates the ubiquitination of c-MYC through FBXW7. a, b Western blot and quantitative PCR assays were performed to examine the protein and mRNA expression of c-MYC and FBXW7 in TRIP13-knockdown U87MG and LN229 cells. c The FBXW7 promoter regions were ligated into the pGL3 plasmid and co-transfected with pRL-TK/shTRIP13/TRIP13. Luciferase activity was examined 48 h after transfection. The pGL3-basic vector was used as the negative control. d A total of six sets of primers were designed within the human FBXW7 promoter, and a ChiP assay was performed by using Flag antibodies. IgG was used as the negative control. e, f Western blot and MTT experiments were performed to assess FBXW7 knockdown in LN229 and U87MG cells with knocked-down TRIP13. All data are shown as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001
