Fig. 2: Low MFN1 level in human HCC was correlated with vascular invasion and poor prognosis.

a qRT-PCR analysis for MFN1 expression levels in 34 HCC samples between tumour and peritumour mesenchyme tissues indicated that MFN1 expression was greatly inhibited in tumour tissues compared with para-tumour tissues. n = 6, mean ± SEM, ***P < 0.001, **P < 0.01, *P < 0.05. b qRT-PCR analysis for MFN1 expression levels in 20 HCC patients with and without portal vein tumour thrombosis (PVTT) indicated that for patients with PVTT MFN1 expression decreased. n = 6, mean ± SEM, ***P < 0.001, **P < 0.01, *P < 0.05. c Representative immunohistochemical (IHC) staining images of MFN1 in HCC tissue microarrays. Scale bar: 100 µm. d, e Kaplan–Meier analysis of overall survival (OS) and disease-free survival (DFS) in HCC patients classified by expression levels of MFN1 showed that MFN1 low expression contributes to poor prognosis.