Fig. 1: Mechanism of the dominant effect of mutant ATG4B (ATG4BC74A).
From: Autophagy as a therapeutic target in pancreatic cancer
Unlike normal ATG4B, ATG4BC74A is devoid of catalytic activity and thus unable to process LC3 to LC3-I, but remains bound to unprocessed LC3, thus functionally sequesters it and prevents autophagosome closure and thus inhibits autophagy.
