Fig. 4: Expression of ITIH5 correlates with reduced liver metastasis in vivo and in human PDAC.
From: Suppression of pancreatic cancer liver metastasis by secretion-deficient ITIH5
a Representative images of IHC expression and gross liver images of ITIH5 in control (Con), ITIH5 (ITI) and ITIH5Δs (Δs) expressing PDAC liver metastases. b Quantification of photomicrographs of IHC images from in vivo samples shown in (a) (n = 3). The control group showed frequent low-intensity (0+, 1+) staining, whereas ITI and Δs showed high-intensity (2+, 3+) staining. c Representative images of IHC expression for ITIH5 and gross in vivo liver images from tumours expressing full-length ITIH5. Small metastases (small met) expressed higher ITIH5. In contrast, large met had lower (or lost) ITIH5 expression. d Representative image of one slide from TMA containing matched human primary and metastatic PDAC and stained for ITIH5 using IHC. ITIH5 expression is generally low in both primary and metastatic PDAC. Focal areas of nonspecific staining of necrotic tissue are also visible. e Representative high-magnification images of ITIH5 IHC in normal human pancreas, primary tumour and matched liver metastasis. f Quantification of ITIH5 in normal pancreas, primary PDAC and PDAC metastases from TMA samples.
