Table 1 Stability and toxicology study of Enpen and TriCurin-Enpen creams. (A) 3.5 months test for Tricurin-EnPen cream. (B) Toxicology study of EnPen: MTT assay % viability. (C) Toxicology study of TriCurin-EnPen: MTT assay % viability.

From: A novel cancer preventative botanical mixture, TriCurin, inhibits viral transcripts and the growth of W12 cervical cells harbouring extrachromosomal or integrated HPV16 DNA

(A) 3.5 months Test for TriCurin-EnPen cream

Concentration (µM)

EnPen alone

TriCurin-EnPen

T (EnPen-TriCurin-EnPen)*

 

Mean

SD

% of Viability

Mean

SD

% of Viability

T statistics

P value

0

1.672

0.108

100

1.665

0.218

100

0.076

0.940

1

1.333

0.279

80

1.172

0.312

70

1.018

0.326

2

1.031

0.336

62

0.880

0.310

52

0.874

0.397

4

0.892

0.240

53

0.478

0.223

29

3.343

0.005

8

0.722

0.182

43

0.313

0.105

19

5.15

0.000

16

0.665

0.200

40

0.120

0.033

7

7.113

0.000

32

0.588

0.151

35

0.021

0.010

1

9.913

0.000

(B) Toxicology study of EnPen: MTT assay % viability

Results

Two-tailed T test statistics

Time (h)

EnPen, mean ± SD

Gynol II, mean ± SD

1% Triton X-100, mean ± SD

EnPen vs. Gynol II

EnPen vs. 1% Triton X-100

Gynol II vs. 1% Triton X-100

T-s*

P-v**

T-s*

P-v**

T-s*

P-v**

0

100.00 ± 5.76

100.00 ± 5.76

100.00 ± 5.76

0.000

1.000

0.000

1.000

0.000

1.000

1

99.04 ± 7.66

78.13 ± 2.11

62.49 ± 17.10

3.722

0.020

2.759

0.051

1.284

0.269

4

105.00 ± 0.96

67.11 ± 6.69

4.71

7.928

0.001

73.871

0.000

6.595

0.022

24

98.06 ± 3.46

3.64 ± 1.03

1.87 ± 0.24

36.988

0.000

39.222

0.000

2.367

0.077

(C) Toxicology study of TriCurin-EnPen: MTT assay % viability

Time (h)

M-EnPen, mean ± SD

TriCurin-EnPen, mean ± SD

Gynol II, mean ± SD

Vagisil (anti-itch cream), mean ± SD

T test (EnPen vs. Vagisil)

T statistics

P value

0

100.00 ± 3.02

100.00 ± 3.02

100.00 ± 3.02

100.00 ± 3.02

0.000

1.000

1

101.33 ± 1.20

N/A

75.51

102.60 ± 2.45

0.658

0.546

4

76.50 ± 4.20

N/A

25.48

50.72 ± 1.89

7.916

0.001

24

79.75 ± 2.30

67.49

3.38

2.48 ± 0.09

47.598

0.000

  1. (A) Enpen and TriCurin-Enpen were stored in the dark at 4 °C for 3.5 months. HeLa cells were exposed to increasing concentrations of Enpen or TriCurin-Enpen for 96 h and the number of viable cells was determined by the MTT assay, as described in the “Methods” section.
  2. *Two-tailed T test.
  3. (B,C) Organotypic human vaginal-ectocervical (VEC) tissue (EpiVaginal, MaTtek, MA) was used to test the toxicity of:
  4. (B) Enpen compared to two positive control creams known to be toxic: Gynol II, 1% Triton X-100, as described in the “Methods” section.
  5. *T-s: T statistics; **P-v: p value.
  6. (C) TrCurin-Enpen and Enpen cream compared to two positive control creams known to be toxic Vagisil (benzocaine 5%, resorcinol 2%) and Gynol II (nonoxynol-9 (3%), as described in the “Methods” section.
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