Fig. 4: Selective reduction of mRNA translation following 4 h SBI-756 treatment.

a Polysome traces of OCI-LY1 cells treated with SBI-756 or without (vehicle) for 4 h. Results are representative of three independent samples. b Quantification of puromycin incorporation in OCI-LY1 cells treated with vehicle, MLN0128 (30 nM), rapamycin (10 nM) or SBI-756 250 nM for 4 h with addition of puromycin to the media for the last 20 min. c Genome-wide transcription and translation mRNA profiling of 25 million cells with or without SBI-756 treatment. Results are from two independent studies. Total mRNA and purified fractions containing four or more bound ribosomes were sequenced using Illumina HiSeq 4 single read. Volcano plots represent differences in transcription (left) and translation (right). Transcription and translation parameters were p ≤ 0.05 and −1.0 ≤ log2 ≥ 1.0. Blue dots identify mRNAs significantly changed in abundance, and red dots identify mRNAs not significantly changed. Statistical analysis was performed using the limma R package via RIVET (Ernlund AW et al., BMC Genomics 2018). d Venn diagram comparing significant differences in transcription and translation. e The top molecular functions of mRNAs significantly down (top) or upregulated (bottom) in translation. f The top cellular components of mRNAs significantly down (top) or upregulated (bottom) in translation.