Fig. 3: USP10 and SYK physically associate and genetic knockdown or knockout of USP10 leads to SYK degradation.
From: Inhibition of the deubiquitinase USP10 induces degradation of SYK
a Immunoprecipitation/immunoblots: Co-immunoprecipitation of SYK protein and USP10 protein in Ba/F3-SYK-TEL cells and co-immunoprecipitation of FLT3 protein and USP10 protein in Ba/F3-FLT3-ITD-luc + cells. b Purified GST-SYK protein was incubated with His-USP10 coupled to NI-NTA beads. Proteins retained on NI-NTA beads were then blotted with indicated antibodies. c Immunoblots: Effects of Crispr knockout of USP10 on SYK protein levels in MOLM14-Cas9-GFP #15 monoclonal cells and effects of Crispr knockout of USP7 on SYK protein levels in MOLM14-Cas9-GFP #8 monoclonal cells. Shown in c are densitometry values for SYK, USP10 and USP7 shown relative to GAPDH. d Immunoblots: Effects of USP10 KD on SYK, FLT3, MAPK and AKT expression in MOLM14 cells. e Measurement of MOLM14 SCR shRNA and MOLM14 USP10 shRNA cell growth by CellTiter Glo following 24 h after seeding. Error bars represent standard deviations. f USP10 shRNA KD in HEL cells. Shown in f are densitometry values for SCR versus USP10 shRNAs, shown relative to GAPDH.
