Table 1 Combination indices generated by Calcusyn software for crenolanib ± C598-0556, crenolanib ± C673-0105, midostaurin ± C598-0556 and midostaurin ± C673-0105 tested against Ba/F3-SYK-TEL, Ba/F3-FLT3-ITD and Ba/F3-FLT3-ITD + SYK-TEL cells.

From: Inhibition of the deubiquitinase USP10 induces degradation of SYK

Cell line

Days of treatment

Drug combination

ED25

ED50

ED75

ED90

CI value

Ba/F3-SYK-TEL

2 days

crenolanib + C598-0556

0.92

1.00

1.08

1.19351

 

Ba/F3-SYK-TEL

2 days

crenolanib + C673-0105

0.83

0.90

0.99

1.09124

 

Ba/F3-SYK-TEL

3 days

crenolanib + C598-0556

0.93

1.03

1.15

1.29099

0.00

Ba/F3-SYK-TEL

3 days

crenolanib + C673-0105

0.68

0.77

0.90

1.0444

0.25

Ba/F3-SYK-TEL

3 days

midostaurin + C598-0556

0.94

1.01

1.08

1.15254

0.50

Ba/F3-SYK-TEL

2 days

midostaurin + C673-0105

0.80

0.88

0.95

1.03945

0.75

Ba/F3-SYK-TEL

3 days

midostaurin + C598-0556

0.89

0.95

1.01

1.07668

1.00

Ba/F3-SYK-TEL

3 days

midostaurin + C673-0105

0.67

0.74

0.81

0.90019

1.25

Ba/F3-FLT3-ITD

2 days

crenolanib + C598-0556

0.48

0.55

0.63

0.72655

1.50

Ba/F3-FLT3-ITD

2 days

crenolanib + C673-0105

0.55

0.62

0.71

0.81216

 

Ba/F3-FLT3-ITD

3 days

crenolanib + C598-0556

0.31418

0.37855

0.4561

0.54956

 

Ba/F3-FLT3-ITD

3 days

crenolanib + C673-0105

0.36445

0.45081

0.55764

0.68978

 

Ba/F3-FLT3-ITD

2 days

midostaurin + C598-0556

0.54678

0.65506

0.78506

0.9412

 

Ba/F3-FLT3-ITD

2 days

midostaurin + C673-0105

0.57086

0.68038

0.81145

0.96839

 

Ba/F3-FLT3-ITD

3 days

midostaurin + C598-0556

0.63815

0.73788

0.8532

0.98654

 

Ba/F3-FLT3-ITD

3 days

midostaurin + C673-0105

0.78463

0.85346

0.93045

1.01679

 

Ba/F3-FLT3-ITD + SYK-TEL

2 days

crenolanib + C598-0556

0.67777

0.76846

0.87128

0.98786

 

Ba/F3-FLT3-ITD + SYK-TEL

2 days

crenolanib + C673-0105

1.01059

1.04929

1.09134

1.13705

 

Ba/F3-FLT3-ITD + SYK-TEL

3 days

crenolanib + C598-0556

0.80774

0.90974

1.02484

1.15475

 

Ba/F3-FLT3-ITD + SYK-TEL

3 days

crenolanib + C673-0105

0.79295

0.88725

0.99281

1.11095a

 

Ba/F3-FLT3-ITD + SYK-TEL

2 days

midostaurin + C598-0556

0.78454

0.94376

1.1353

1.3657a

 

Ba/F3-FLT3-ITD + SYK-TEL

2 days

midostaurin + C673-0105

0.65125

0.77988

0.93409

1.11899

 

Ba/F3-FLT3-ITD + SYK-TEL

3 days

midostaurin + C598-0556

0.58832

0.70615

0.84757

1.01732

 

Ba/F3-FLT3-ITD + SYK-TEL

3 days

midostaurin + C673-0105

0.81731

0.9078

1.01196

1.13232

 
  1. Proliferation assays/combination studies were carried out for 2 and 3 days, respectively.
  2. aCombination indices were calculated based on four data points. Combination indices for all other studies were calculated based on five data points.
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