Fig. 2: miR-27a affects overall mitochondrial activities.

a Kinetic profile of the Oxygen Consuming Rate (OCR) assay in HCT116 and SW480 miR-27a_KD and HT29 miR-27a_OE cells and their relative CTRL_KD and CTRL_OE cells, respectively. OCR, expressed as picomoles of oxygen/minute, was measured in real time under basal conditions and in response to the indicated compounds: Oligomycin, FCCP, Rotenone and Antimycin A. Data are reported as mean ± SEM of experiments performed in triplicate; the means values were compared by one-way analysis of variance test (ANOVA). Statistical significance was considered when *p ≤ 0.05, **p ≤ 0.01, ***p < 0.001. The histograms show the values of basal respiration, ATP production and maximal respiration as by the calculations of the experimental OCR data obtained from the various pairs of cell lines analysed. b Immunoblot analysis of PGC-1α, CPT1A and ACAD9 in the same cells as in a. β-Tubulin was used for protein loading normalisation. Data are reported as mean ± SEM of experiments performed in triplicate; the means values were compared by one-way analysis of variance test (ANOVA). Statistical significance was considered when *p ≤ 0.05, **p ≤ 0.01, ***p < 0.001.