Fig. 2: Characterisation of the primary culture of the tumour cells derived from miPS-Huh7cm cells injected into the liver.
From: A novel model of liver cancer stem cells developed from induced pluripotent stem cells
a Representative cluster analysis among miPS, miPS-Huh7cm cells and miPS-Huh7cm P cells (left). The colour range represents the log10(FPKM+1) value from large (red) to small (blue). Main oncogenes and CSC markers implicated in liver cancer are enlarged (right). b Stemness marker gene mRNA expression levels were analysed through RT-qPCR analysis in miPS-Huh7cm cells, and miPS-Huh7cm P cells were compared to miPS (*p < 0.01, **p < 0.001, ***p < 0.0001, ****p < 0.00001). c Liver cancer stem cell marker gene mRNA expression levels were analysed through RT-qPCR analysis in miPS-Huh7cm cells, and miPS-Huh7cm P cells were compared to miPS (**p < 0.001, ***p < 0.0001, ****p < 0.00001). d Flow cytometric analysis for liver cancer-associated markers such as GPC3, AFP and CEA with Nanog in miPS-Huh7cmP1 cells. Each result is shown as a representative of at least five independent experiments.
