Fig. 4: Self-renewal potential of the CSC-like cells.
From: A novel model of liver cancer stem cells developed from induced pluripotent stem cells
a Representative images for adherent culture of Huh7, miPS-Huh7cm and miPS-Huh7cmP1 cells. Scale bars represent 100 μm. b FACS analysis shows GFP population in miPS-Huh7cm and miPS-Huh7cmP1 cells. c Sphere-formation assay in serum-free medium shows spherogenic potential of both miPS-Huh7cm and miPS-Huh7cmP1 cells compared to Huh7 cells. Scale bars: 50 μm. d Number of spheres formed from miPS-Huh7cmP1 cells compared to miPS-Huh7cm and Huh7 cells. e Extreme limiting dilution assay assessment of the limiting dilution sphere-forming potential of miPS-Huh7cm and miPS-huh7cmP1 cells. f Immunofluorescence staining for Nanog and Oct 3/4 in tumour spheroid derived from miPS-Huh7cm and miPS-Huh7cmP1 cells. Scale bars represent 64 μm.
