Fig. 1: Expression of key enzymes of SSP under normoxic and hypoxic conditions.
From: Serine-dependent redox homeostasis regulates glioblastoma cell survival
a–b Gene expression (upper panel) and protein levels (lower panel) of the SSP enzymes PHGDH (a), SHMT1 and 2 (b) in breast cancer (MDA-MB-231 and MDA-MB-468) and glioma (LN-308, LN-428, LNT-229, G55 and LN-464) cell lines were investigated under standard conditions (DMEM containing 10% FCS and 25 mM glucose under normoxia) by qPCR and immunoblot. Values are normalised to 18 S as well as SDHA housekeeping gene expression (n = 3, mean ± SD). Cellular lysates were analysed by immunoblot with antibodies for PHGDH, SHMT1, SHMT2 and actin. c Gene expression of the SSP enzymes PHGDH, SHMT1 and 2 in glioma cell lines were investigated under starvation conditions (24 h in serum-free medium and 1% oxygen (O2)). Gene expression was measured by qPCR. Values are normalised to 18 S as well as SDHA housekeeping gene expression (n = 3, mean ± SD). Significant gene induction (*p < 0.05 or **p < 0.01) is illustrated by green boxes, significant gene suppression (*p < 0.05 or **p < 0.01) is illustrated by red boxes and no significant change in gene expression is illustrated by yellow boxes.
