Fig. 3: Serine restriction sensitises for hypoxia-induced cell death and increases intracellular ROS levels.
From: Serine-dependent redox homeostasis regulates glioblastoma cell survival
a–c LN-308, LNT-229 and G55 cells were exposed to glucose restricted (2 mM glucose) serum- and glycine-free DMEM under normoxic or hypoxic (0.1% oxygen (O2)) conditions with or without CBR-5884 as indicated. Serine was replenished as indicated. a Cell death was quantified by LDH-release (n = 4, mean ± S.D., n.s. not significant, *p < 0.05, **p < 0.01). b Reactive oxygen species (ROS) levels were measured by H2DCFDA-FACS (n = 3, mean ± SD, n.s. not significant, **p < 0.01). c Analysis of NADPH/NADP+ ratios was performed by a luminescence-based assay (n = 3, mean ± SD, n.s. not significant, *p < 0.05).
