Fig. 4: Distinct immune microenvironment of OPC subtypes revealed by multiplex IHC.

a The representative multiplex IHC image of OPC tumour. The representative tumour nest and stroma sites were selected, and the density of CD8⁺ T cells and CD68⁺ macrophages, as well as the proportion of CD73⁺ tumour cells were counted both in tumour nests and stroma. b The representative multiplex IHC images of subtypes of OPC tumours. c The dot plots of immune cell densities of OPC tumours, total CD8⁺ T cells, PD-1⁺CD8⁺ T cells and CD68⁺ macrophages both in tumour nests and stroma. d The ratio of CD8⁺ T cell density in tumour nest to CD8⁺ T cell density in stroma in the three subtypes (left panel). The f-TBRS score of the three subtypes in 37 YOPC tumours (right panel). e The representative CD73 IHC image of two XB type tumours (YOPC8 and YOPC32, upper panel). The dot plot of the proportion of CD73⁺ tumour cells in the subtypes (lower panel). The cell densities (c) and f-TBRS (d) were compared using one-way ANOVA with Bonferroni post hoc test. The statistically significant results per post hoc analysis are indicated with asterisks (*p < 0.05).