Fig. 1: Circulating tumor cell detection by immunofluorescence staining and in spike-in experiments.

a A representative immunofluorescence (IF) staining image of NPC CTCs after enrichment. b the positive predictive value (PPV), sensitivity, and the false discovery rate (FDR) of cell line spike-in experiments with KYSE270 and blood from a healthy individual (RC2202) at concentrations of 100%, 10%, 5%, 2.5% and 1.25% of the DNA content (All mutations detected by Mutect 1).