Table 4 Results of univariate analysis of PD-L1, PD-1, CD8, CD4 and CD3 in relation to durable clinical benefit (DCB) are shown for biopsies taken before chemotherapy (BCT) and biopsies taken after the last line of chemotherapy (ACT).

Tumour PD-L1 expression

0.88

Tumour PD-L1 expression

0.013

 <1% (n = 52)

21

1

<1% (n = 47)

23

1

 1–49% (n = 7)

14

0.62 (0.068–5.7)

1–49% (n = 9)

22

0.94 (0.17–5.2)

 ≥50% (n = 8)

25

1.2 (0.22–7.0)

≥50% (n = 13)

69

7.4 (1.9–28.6)

Stromal infiltration of PD-L1⁺ IC

0.60

Stromal infiltration of PD-L1⁺ IC

0.22

 Low infiltration (n = 39)

26

1

Low infiltration (n = 45)

24

1

 Intermediate infiltration (n = 15)

13

0.45 (0.085–2.3)

Intermediate infiltration (n = 14)

43

2.3 (0.66–8.2)

 High infiltration (n = 11)

18

0.64 (0.12–3.5)

High infiltration (n = 8)

50

3.1 (0.66–14.5)

Stromal infiltration of PD-1⁺ IC

0.55

Stromal infiltration of PD-1⁺ IC

0.006

 Low infiltration (n = 40)

25

1

Low infiltration (n = 41)

17

1

 Intermediate infiltration (n = 17)

12

0.40 (0.078–2.1)

Intermediate infiltration (n = 17)

41

3.4 (0.96–12.0)

 High infiltration (n = 9)

22

0.86 (0.15–4.8)

High infiltration (n = 12)

67

9.7 (2.3–41.4)

Tumour infiltration of CD8⁺ IC

0.52

Tumour infiltration of CD8⁺ IC

0.009

 Low infiltration (n = 37)

22

1

Low infiltration (n = 39)

18

1

 Intermediate infiltration (n = 11)

9

0.36 (0.040–3.3)

Intermediate infiltration (n = 16)

31

2.1 (0.55–7.9)

 High infiltration (n = 18)

28

1.4 (0.38–5.1)

High infiltration (n = 16)

63

7.6 (2.1–28.0)

Stromal infiltration of CD8⁺ IC

0.74

Stromal infiltration of CD8⁺ IC

0.044

 Low infiltration (n = 11)

18

1

Low infiltration (n = 18)

17

1

 Intermediate infiltration (n = 28)

18

0.98 (0.16–6.0)

Intermediate infiltration (n = 17)

18

1.1 (0.19–6.2)

 High infiltration (n = 27)

26

1.6 (0.27–9.1)

High infiltration (n = 35)

46

4.2 (1.0–17.2)

Stromal infiltration of CD3⁺ IC

0.20

Stromal infiltration of CD3⁺ IC

0.035

 Low infiltration (n = 23)

9

1

Low infiltration (n = 26)

19

1

 Intermediate infiltration (n = 26)

31

4.7 (0.88–24.9)

Intermediate infiltration (n = 23)

26

1,5 (0.39–5.7)

 High infiltration (n = 17)

24

3.2 (0.52–20.2)

High infiltration (n = 20)

55

5.1 (1.4–19.1)

Stromal infiltration of CD4⁺ IC

0.31

Stromal infiltration of CD4⁺ IC

0.10

 Low infiltration (n = 16)

19

1

Low infiltration (n = 15)

20

1

 Intermediate infiltration (n = 19)

11

0.51 (0.074–3.5)

Intermediate infiltration (n = 17)

18

0.86 (0.15–5.1)

 High infiltration (n = 31)

29

1.8 (0.41–7.8)

High infiltration (n = 37)

43

3.0 (0.74–12.6)

Combined tumour and stromal infiltration of CD8⁺ IC

0.64

Combined tumour and stromal infiltration of CD8⁺ IC

0.008

 Low infiltration of tumour and stromal CD8⁺ IC (n = 28)

21

1

Low infiltration of tumour and stromal CD8⁺ IC (n = 31)

19

1

 High infiltration of tumour or stromal CD8⁺ IC (n = 20)

15

0.65 (0.14–3.0)

High infiltration of tumour or stromal CD8⁺ IC (n = 11)

9

0.42 (0.044–3.9)

 High infiltration of tumour and stromal CD8⁺ IC (n = 18)

28

1.4 (0.36–5.6)

High infiltration of tumour and stromal CD8⁺ IC (n = 28)

54

4.8 (1.5–15–3)

Combined stromal infiltration of PD-1⁺ and CD8⁺ IC

0.62

Combined stromal infiltration of PD-1⁺ and CD8⁺ IC

0.001

 Low infiltration of stromal PD-1⁺ and CD8⁺ IC (n = 31)

19

1

Low infiltration of stromal PD-1⁺ and CD8⁺ IC (n = 30)

20

1

 High infiltration of stromal PD-1⁺ or CD8⁺ IC (n = 17)

29

1.7 (0.44–6.8)

High infiltration of stromal PD-1⁺ or CD8⁺ IC (n = 15)

7

0.29 (0.031–2.6)

 High infiltration of stromal PD-1⁺ and CD8⁺ IC (n = 18)

17

0.83 (0.18–3.8)

High infiltration of stromal PD-1⁺ and CD8⁺ IC (n = 24)

63

6.7 (2.0–22.5)

Combined stromal infiltration of PD-L1⁺ and CD8⁺ IC

0.44

Combined stromal infiltration of PD-L1⁺ and CD8⁺ IC

0.043

 Low infiltration of stromal PD-L1⁺ and CD8⁺ IC (n = 31)

19

1

Low infiltration of stromal PD-L1⁺ and CD8⁺ IC (n = 30)

17

1

 High infiltration of stromal PD-L1⁺ or CD8⁺ IC (n = 15)

33

2.1 (0.52–8.4)

High infiltration of stromal PD-L1⁺ or CD8⁺ IC (n = 20)

35

2.7 (0.71–10.2)

 High infiltration of stromal PD-L1⁺ and CD8⁺ IC (n = 19)

16

0.78 (0.17–3.6)

High infiltration of stromal PD-L1⁺ and CD8⁺ IC (n = 17)

53

5.6 (1.5–21.8)