Table 1 Mutagenesis primers.
Forward | Reverse | |
|---|---|---|
AKT1 D323H | gccgtagtcattgtgctccagcacctcgg | ccgaggtgctggagcacaatgactacggc |
AKT1 D292A | cttgcacagcccgaaggctgtgatcttaatgtgc | gcacattaagatcacagccttcgggctgtgcaag |
AKT1 W80A | cgatgacagtggtcgcctgcaggcagcgga | tccgctgcctgcaggcgaccactgtcatcg |
AKT1 Q79K | gacagtggtccacttcaggcagcggatga | tcatccgctgcctgaagtggaccactgtc |
AKT1 M227Q | ccgttggcgtactcctggacaaagcagaggcg | cgcctctgctttgtccaggagtacgccaacgg |
AKT2 W80A | ctcgatgactgtggtcgcctgcaggcagcgtatg | catacgctgcctgcaggcgaccacagtcatcgag |
