Fig. 3: Ref-1 and STAT3 pathway inhibitors blocked proliferation and colony formation of MPNST cells. | British Journal of Cancer

Fig. 3: Ref-1 and STAT3 pathway inhibitors blocked proliferation and colony formation of MPNST cells.

From: Exploring transcriptional regulators Ref-1 and STAT3 as therapeutic targets in malignant peripheral nerve sheath tumours

Fig. 3

Alamar blue assay was used to determine dose response curves of various MPNST cell lines to Ref-1 inhibitors (a–c) with IC50s noted. Soft agar colony assays (d) demonstrate that Ref-1 inhibitors blocked the number of ST88-14 colonies formed (e; scale bar 0.5 mm) and resulted in smaller colonies (f, ***P < 0.001; scale bar 0.125 mm). Alamar blue assay following treatment with STAT3 inhibitors, Ruxolitinib (g) and Napa (h) in MPNST cells, n = 3 ± SE. Rux and Napa blocked the activation of STAT3 in NF90-8 cells as determined by western blot in (i).

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