Fig. 1: ETNK2 is upregulated in human GC cell lines and promotes malignant behaviours. | British Journal of Cancer

Fig. 1: ETNK2 is upregulated in human GC cell lines and promotes malignant behaviours.

From: Hepatic metastasis of gastric cancer is associated with enhanced expression of ethanolamine kinase 2 via the p53–Bcl-2 intrinsic apoptosis pathway

Fig. 1

a qRT-PCR analysis of ETNK2 mRNA levels in human GC cell lines. b CRISPR/Cas9-mediated knockout (KO) of ETNK2 in MKN1 cells. Upper panel: DNA sequencing of the PCR product encompassing the ETNK2 exon indicates successful base deletion in MKN1 KO cells. Lower panel: western blot analysis of ETNK2 protein expression in two MKN1 ETNK2 KO cell lines. c Spearman’s correlation analysis of 84 cancer-related genes with ETNK2 in GC cell lines. The levels of AHNAK and TGFB1 significantly correlate with that of ETNK2. d The fold changes of transcripts identified in PCR array analysis in ETNK2 KO cells. e Proliferation of parental and ETNK2 KO MKN1 cell lines. f Images (left) and quantification (right) of Matrigel invasion assays of parental and ETNK2 KO MKN1 cells. g Images (left) and quantification (right) of wound-healing migration assays of parental and ETNK2 KO cell lines. *P < 0.005. Data are presented as the mean ± standard deviation.

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