Fig. 3: ETNK2 knockout promotes cell cycle arrest and apoptosis of GC cells.

a Fluorescence and phase contrast micrographs of untransfected and ETNK2 KO MKN1 cells stained with annexin V (red) to detect apoptotic cells. Irradiated cells served as a positive control. b Flow cytometric dot plots (upper) and quantification (lower) of untransfected and ETNK2 KO MKN1 cells stained to detect cells with mitochondrial membrane potential depolarisation. c Flow cytometric dot plots (upper) and quantification (lower) of untransfected and ETNK2 KO MKN1 cells stained with anti-caspase antibody. d Western blot analysis of apoptosis-related proteins in untransfected and ETNK2 KO MKN1 cells. β-Actin served as a loading control. e Light micrographs (left) and quantification (right) of untransfected and ETNK2 KO MKN1 cells stained with Redox dye to detect cells in G0/G1, S, and G2/M phases of the cell cycle. *P < 0.005. Data are presented as the mean ± standard deviation.