Fig. 1: Olaparib in combination with whole thoracic radiation causes normal tissue toxicity. | British Journal of Cancer

Fig. 1: Olaparib in combination with whole thoracic radiation causes normal tissue toxicity.

From: Olaparib increases the therapeutic index of hemithoracic irradiation compared with hemithoracic irradiation alone in a mouse lung cancer model

Fig. 1

a Experimental schematic for the tolerability test: A/J mice were given vehicle or olaparib (100 mg/kg, p.o.) 1 h prior to and 2 h after 13 Gy radiation on the whole thorax. Mice were euthanised 2 weeks after the treatment and BrdU was injected 1.5 h before euthanasia. Oesophagi were collected for H&E or IHC staining. b Mouse body weight change was monitored and plotted over 2 weeks time after the treatment. The plot showed that olaparib combined with whole thoracic radiation caused body weight loss. c Representative H&E staining of oesophageal tissue. Arrows delineate IEL (inner oesophageal layer) thickness. Epithelial hyperplasia and hypertrophy were circled black. Inflammatory cell infiltration circled green. d Thickness of the inner oesophageal layer was measured at four representative spots of four different cross-section regions of each oesophagus (n = 3/group). e Representative BrdU staining of oesophageal tissue. f Quantification of BrdU+ basal cells in oesophagus epithelial layer. A minimum of 400 basal cells were counted. Graph represents mean ± SEM; *P ≤ 0.05; n.s. not significant. Scale bars: 50 μm.

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