Fig. 2: Early intervention with olaparib and hemithorax radiation does not increase radiation-induced body weight loss or lung fibrosis. | British Journal of Cancer

Fig. 2: Early intervention with olaparib and hemithorax radiation does not increase radiation-induced body weight loss or lung fibrosis.

From: Olaparib increases the therapeutic index of hemithoracic irradiation compared with hemithoracic irradiation alone in a mouse lung cancer model

Fig. 2

a Experimental schematic for the early intervention study: A/J mice were i.p. injected with urethane to induce lung tumour development. After 8 weeks, mice were given olaparib (50 mg/kg, p.o.) or vehicle 1 h prior to 5 Gy hemithoracic radiation on the left side. The treatment was repeated five cycles. Mice were euthanised 24 weeks after the initiation of treatment. b Body weight changes of mice in four groups were recorded and plotted after treatment initiation. n = 3–9/group. c Representative Masson’s Trichrome staining of left lung tissue, with collagen fibres dyed blue. d Lung fibrosis was scored using the modified Ashcroft system on a scale of 0–8. Symbols represent individual animals within each treatment group. e α-SMA IHC staining of left lung sections. f Quantification of α-SMA-positive area in left lung sections. All data are expressed as mean ± SEM. **P < 0.01; n.s. not significant. Scale bars: 100 µm.

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