Fig. 2: SLC2A5 promotes CRC cells invasion and migration in vitro. | British Journal of Cancer

Fig. 2: SLC2A5 promotes CRC cells invasion and migration in vitro.

From: S100P contributes to promoter demethylation and transcriptional activation of SLC2A5 to promote metastasis in colorectal cancer

Fig. 2

a SLC2A5 protein and mRNA expression of CRC cell lines were measured by western blotting (upper) and qRT-PCR (lower). Results are shown as mean ± SEM (n = 3). b Transfection efficiency was detected by western blotting (upper) and qRT-PCR (lower). Results are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, based on Student’s t-test. c In SW480 and Caco2 cells that stably overexpressed SLC2A5 (LV-SLC2A5) or control vector (LV-NC), migration ability was measured by wound healing assays. Scale bar, 100 μm (10×). Results are presented as mean ± SEM (n = 3). ***P < 0.001, based on Student’s t-test. d In SW480 and Caco2 cells that stably expressed LV-SLC2A5 or LV-NC, migration and invasion abilities were measured by transwell assays. Scale bar, 50 μm (20×). Results are presented as mean ± SEM (n = 3). ***P < 0.001, based on Student’s t-test. e In HCT116 and RKO cells that stably knocked down SLC2A5 (sh-SLC2A5) or control vector (sh-NC), migration ability was measured by wound healing assays. Scale bar, 100 μm (10×). Results are presented as mean ± SEM (n = 3). ***P < 0.001, based on Student’s t-test. f In HCT116 and RKO cells that stably expressed sh-SLC2A5 or sh-NC, migration and invasion abilities were measured by transwell assays. Scale bar, 50 μm (20×). Results are presented as mean ± SEM (n = 3). ***P < 0.001, based on Student’s t-test.

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