Fig. 3: SLC2A5 promotes CRC cells metastasis in vivo. | British Journal of Cancer

Fig. 3: SLC2A5 promotes CRC cells metastasis in vivo.

From: S100P contributes to promoter demethylation and transcriptional activation of SLC2A5 to promote metastasis in colorectal cancer

Fig. 3

a The endogenous expression levels of SLC2A5 in MC38 and CT26 cells were detected by western blotting. b Protein expression levels of SLC2A5 in MC38 and CT26 cells that expressed LV-SLC2A5 or LV-NC were detected using western blotting. c Intrasplenic injection model was used to evaluate the in vivo effects of SLC2A5 on tumour metastasis. Representative images of livers derived from C57BL/6 and BALB/c mice injected with LV-SLC2A5 or LV-NC transfected MC38 or CT26 cells are shown. Formation of metastatic nodules at the liver surfaces are summarised in the right panels. Results are presented as mean ± SEM (n = 6). ***P < 0.001, based on Student’s t-test. d Representative images of H&E-stained sections derived from the liver metastatic nodules. The sections of liver derived from mice that injected with LV-NC cells were used as control. Scale bar, 200 μm (4×), 20 μm (40×). e Representative IHC images of SLC2A5 expression in the liver sections. Scale bar, 20 μm (40×). f Survival curves of mice in different groups.

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