Fig. 5: Exogenous amino acid supplementation inhibits plasma-mediated oxidative stress and viability.
From: The amino acid metabolism is essential for evading physical plasma-induced tumour cell death
a representative images and quantification of viability (sytox green), cellular ROS (CellROX green) and mitochondrial membrane potential (TMRE) of plasma-treated Panc-1 cells supplemented with exogenous glutamine, valine or tyrosine (200 μM). Scale bar is 100 μm. b representative images and quantification of viability (sytox green) in Panc-1 derived spheroids supplemented with exogenous glutamine, valine or tyrosine (200 μM). Scale bar is 500 μm. Data are mean ± SEM derived from three independent experiments with *p < 0.05, **p < 0.01 and ***p < 0.001. Statistical analysis was done using one-way ANOVA with Tukey post-test for multiple comparisons.
