Fig. 2: T cells prevent metastasis.
From: CD8+ T cells inhibit metastasis and CXCL4 regulates its function
In mice with no T, B or NK cells (BALB/c NOD/scid), non-metastatic 67NR breast cancer cells metastasise to the lung from the mammary fat pad similar to metastatic 4T1 cells (a–h). a A cartoon showing the mammary fat pad injection (top). Representative images of whole-body bioluminescence of NOD/scid mice harbouring 67NR and 4T1 tumours (middle) and of tumours excised from these mice (bottom) (n = 5). b The mean tumour volume of the tumours excised from these mice (n = 5). c Representative H&E staining of 67NR and 4T1 tumours from BALB/c NOD/scid mice (n = 5). Scale bar: 200 µm. d Representative bioluminescence images of lungs excised from BALB/c NOD/scid mice harbouring 67NR or 4T1 tumours at the orthotopic mammary fat pad (top). e The mean total flux from these lungs (bottom) (n = 5). f The number of visible lung nodules in 67NR and 4T1 tumour-bearing BALB/c NOD/scid mice (n = 5). (g) H&E staining of representative lungs from NOD/SCID mice injected with 67NR and 4T1. Scale bar: 200 µm. h The number of CTCs isolated from BALB/c NOD/scid mice harbouring 67NR and 4T1 tumours at the mammary fat pad (n = 5). In mice with no T cells (BALB/c Nude), non-metastatic 67NR breast cancer cells metastasise to lung from the mammary fat pad similar to metastatic 4T1 cells (i–p). i A cartoon showing the mammary fat pad injection (top). Representative images of whole-body bioluminescence of BALB/c Nude mice harbouring 67NR and 4T1 tumours (middle) and of tumours excised from these mice (bottom) (n = 5). j The mean tumour volume of the tumours excised from these mice (n = 5). k Representative H&E staining of 67NR and 4T1 tumours from BALB/c Nude mice (n = 5). Scale bar: 200 µm. l Representative bioluminescence images of lungs excised from BALB/c Nude mice harbouring 67NR or 4T1 tumours at the orthotopic mammary fat pad (top) and m the mean total flux from these lungs (bottom) (n = 4). n The number of visible lung nodules in 67NR and 4T1 tumour-bearing BALB/c Nude mice (n = 5). o H&E staining of representative lungs from Nude mice injected with 67NR and 4T1. Scale bar: 300 µm. p The number of CTCs isolated from BALB/c Nude mice harbouring 67NR and 4T1 tumours at the mammary fat pad (n = 5). Quantitative data are mean ± SEM. Statistical analyses were performed by unpaired two-tailed Student’s t test; ns indicates not significant, ∗P ≤ 0.05, ∗∗P ≤ 0.01, ∗∗∗∗P ≤ 0.0001.
