Fig. 5: The growth inhibitory effect of 5-ASA on PC/AA/C1 adenoma- and LS174T carcinoma-derived spheroids is sustained for 14 days after treatment is stopped.

a–d PC/AA/C1 human adenoma-derived cells. a Mean number of spheroids per well after 7 days in culture. n = 3 ± SEM; *p < 0.05. b Mean log spheroid area over 21 days in culture. 5-ASA was withdrawn from the culture media on day 7. n = 3 ± SEM. c Log spheroid area after 21 days in culture, demonstrating distribution of spheroid size. n = 1 ± SD (representative of n = 3). d Mean log spheroid area of spheroids at day 21. n = 3 ± SEM; **p < 0.01. e–h LS174T human carcinoma-derived cells. e Mean number of spheroids per well after 7 days in culture. n = 3 ± SEM. f Mean log spheroid area over 21 days in culture. 5-ASA was withdrawn from the culture media on day 7. n = 3 ± SEM. g Log spheroid area after 21 days in culture, demonstrating distribution of spheroid size. n = 1 ± SD (representative of n = 3). h Mean log spheroid area of spheroids at day 21. n = 3 ± SEM; *p < 0.05; **p < 0.01.