Fig. 2: Surface values of immune checkpoints on exosomes isolated from plasma of HNSCC patients. | British Journal of Cancer

Fig. 2: Surface values of immune checkpoints on exosomes isolated from plasma of HNSCC patients.

From: Changes in circulating exosome molecular profiles following surgery/(chemo)radiotherapy: early detection of response in head and neck cancer patients

Fig. 2

Exosomes from n = 17 HNSCC patients obtained at defined time-points were captured using biotinylated CD63-antibodies and stained with fluorochrome-conjugated antibodies against PD-L1 (a), CTLA-4 (b), OX40 (c) and OX40-L (d). Surface values as determined by on-bead flow cytometry are shown as RFI compared to an appropriate isotype control. Results are plotted as box-and-whisker blots representing the median value, the 25th and 75th quartiles and the range. Wilcoxon related samples tests were applied to each compare pre-OP, post-OP, end (C)RT and follow-up. Mann–Whitney test was applied to compare recurrence with follow-up. * or # and ** or ## correspond to p ≤ 0.05 and p ≤ 0.01, respectively. Asterisks and values above boxes indicate the significance compared to pre-OP. Hashtags above boxes indicate the significance compared to follow-up. c RT (chemo)radiotherapy

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