Fig. 5: KLF5 protein regulates CCAT1 expression. | British Journal of Cancer

Fig. 5: KLF5 protein regulates CCAT1 expression.

From: A stem cell marker KLF5 regulates CCAT1 via three-dimensional genome structure in colorectal cancer cells

Fig. 5

a Expression levels of CCAT1 RNA and KLF5 mRNA in KLF5 knockdown cells. The relative value is calculated by the expression level of the si-negative control transfected cells (siNC). *P < 0.01. b Expression levels of KLF5 mRNA and CCAT1 RNA in ML264-treated cells (HT29, SW48). ML264 (10 µM) was treated for 24 h. The relative value is calculated by the expression level of DMSO-treated control cells. *P < 0.01. c The binding of the KLF5, BRD4, MED1 and RAD21 proteins to the CCAT1 TSS region in the HT29 (top) and SW48 (bottom) cell lines analysed by ChIP-qPCR. The fold enrichment normalised to the value of the IgG control is shown. *P < 0.01. d Expression level of CCAT1 RNA in five heterodeletion mutants of the KLF5 enhancer. The relative value is calculated by the expression level of the parental cells. The average value of five heterodeletion mutants is also shown. *P < 0.01.

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