Fig. 4: Current applications of cfDNA analysis.
From: Cell-free DNA analysis in current cancer clinical trials: a review
Current methods for cfDNA analysis go far beyond single-nucleotide substitution mutations, including detection of DNA copy number aberrations, analysis of mutational signatures, detection of chromosomal translocations, calculation of tumour mutational burden, evaluation of tumour heterogeneity through allelic fraction calculation, analysis of methylation patterns and comparison against normal DNA to detect and filter alterations due to clonal haematopoiesis.
