Fig. 2: MBD3 promotes HCC cell growth and metastasis in vitro and in vivo. | British Journal of Cancer

Fig. 2: MBD3 promotes HCC cell growth and metastasis in vitro and in vivo.

From: MBD3 promotes hepatocellular carcinoma progression and metastasis through negative regulation of tumour suppressor TFPI2

Fig. 2

a Western blotting of MBD3 in normal hepatocyte L02 and other five HCC cell lines. b Flag-MBD3 was stably transfected in Hep3B cells and Huh7 cells were stably infected with lentivirus expressing control or MBD3 shRNA (−1 and −2), protein expression levels were analysed by western blotting. c Resistant Flag-MBD3 was stably transfected into Huh7-shMBD3 cells and the indicated protein expression levels were detected by western blotting. d, e The effect of MBD3 on the growth of HCC cells was detected by MTS assay. f, g The effect of MBD3 on the invasion of HCC cells was detected by transwell assay. Scale bar: 100 μm. h, i Representative image of the subcutaneous tumorigenesis in nude mice, tumour weight and tumour growth curve of each group (n = 7/group) were analysed. j Hep3B-control or Hep3B-MBD3 cells were injected into the tail vein of nude mice (n = 3/group) to detect lung metastasis, and the fluorescence intensity from week 2–4 was measured. d, e, j Mean ± SD, two-way ANOVA; f–i (upper panel) Mean ± SD, or h, i (lower panel) Mean ± SEM, unpaired Student’s t-test or Welch’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.

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