Fig. 6: TFPI2 promoter can be deacetylated by the MBD3/NuRD complex.

a The endogenous association of CHD4 and HDAC1 with MBD3 is detected in Huh7 cells. Immunoprecipitation (IP) was performed using anti-MBD3 antibody and the immunoprecipitates (IPs) were probed with indicated antibodies. b Representative fluorescence staining of MBD3, CHD4 and HDAC1 in different parts of the same human HCC tissue. c ChIP assay designed to detect the binding region of MBD3/NuRD complex on TFPI2 promoter. Schematic showing the amplification regions of the ChIP primers (1# to 6#) on TFPI2 promoter. d ChIP assay were performed with antibodies of MBD3, CHD4 and HDAC1 separately using the ChIP primers (1# to 6#), and the IPs were analysed by qPCR. e, f ChIP assays of the indicated proteins binding on TFPI2 promoter in control and MBD3-knockdown Huh7 cells. The IPs were analysed by qPCR (primer #5), assayed by unpaired Student’s t-test or Welch’s t-test.