Fig. 5: FGF1 mediates LHX2-induced proliferation, migration and invasion in an autocrine or paracrine manner.
From: LHX2 facilitates the progression of nasopharyngeal carcinoma via activation of the FGF1/FGFR axis
a–e FGF1 siRNA was transfected into HONE1 cells stably overexpressing LHX2 or the empty vector. Colony-formation assay (a), wound-healing assay (b) and transwell migration and invasion assays (c). Western blot analysis of LHX2, FGF1 and pro-survival signalling pathways (d). Western blot analysis of β-catenin signalling pathway and EMT-related proteins (e). Each experiment was independently repeated at least three times. f, g HONE1 and SUNE1 cells were treated with FGF1-CM or EV-CM for the indicated time. Western blot analysis of pro-survival signalling pathways (f). Western blot analysis of β-catenin signalling pathway and EMT-related proteins (g). Each experiment was independently repeated at least three times. Data shown as mean ± SD. *P < 0.05, **P < 0.01. FGF1-CM FGF1-condition medium, EV empty vector.
